Matthew Alberto Cruz

  • Rice University (2017)

  • Biochemistry, Biophysics, and Structural Biology

  • Gregory R. Bowman, Ph.D.

  • Assay H. sapiens and S. cerevisiae TFIIH homologs for translocase activity and test mechanistic hypotheses using ensemble and single molecule data on the structural dynamics of transcription initiation.



Utilizing biophysical approaches I will measure and compare the translocase activity of eukaryotic TFIIH complexes from H. sapiens and S. cervisiae. These experiments will include steady state measurements of ATP hydrolysis as a function of DNA template length and single molecule TIRF-based fluorescent enhancement measurements. I will also use magnetic tweezers experiments to measure the dynamics of DNA opening stimulated by TFIIH dsDNA translocation using human transcription factors. Additional experiments may include measuring TFIIH-DNA interactions using optical tweezers.

Last Updated: 8/30/2017 8:38:27 PM

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