Matthew Alberto Cruz

  • Rice University (2017)

  • Biochemistry, Biophysics, and Structural Biology

  • Rotating in the lab of Tae Seok Moon, Ph.D.

  • Assay H. sapiens and S. cerevisiae TFIIH homologs for translocase activity and test mechanistic hypotheses using ensemble and single molecule data on the structural dynamics of transcription initiation.

  • matthewcruz@wustl.edu

Research

Utilizing biophysical approaches I will measure and compare the translocase activity of eukaryotic TFIIH complexes from H. sapiens and S. cervisiae. These experiments will include steady state measurements of ATP hydrolysis as a function of DNA template length and single molecule TIRF-based fluorescent enhancement measurements. I will also use magnetic tweezers experiments to measure the dynamics of DNA opening stimulated by TFIIH dsDNA translocation using human transcription factors. Additional experiments may include measuring TFIIH-DNA interactions using optical tweezers.

Last Updated: 8/30/2017 8:38:27 PM

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