Paul C. Bridgman, Ph.D.

Professor
Neuroscience
Biomedical Engineering

Neurosciences Program
Molecular Cell Biology Program
Developmental, Regenerative and Stem Cell Biology Program

  • 314-362-3449

  • 314-362-3391

  • 314-362-3446

  • 8108

  • 927 McDonnell Medical Sciences Building

  • bridgmap@wustl.edu

  • http://conifer.wustl.edu/

  • neurobiology, development, imaging, axon, cytoskeleton

  • Cell biology of developing nerve and muscle cells

Research Abstract:

My laboratory focuses on the basic cellular properties of developing nerve and muscle with emphasis on relating structure to function. Several different projects are currently underway. The first involves the mechanism of nerve growth cone locomotion and the relationship between growth cone motility, guidance and nerve outgrowth. The focus of this work is on the mechanoenzymes and cytoskeletal components that are likely to participate in growth cone motility. Cellular and molecular methods are being used to locate and disrupt the activity of mechanoenzymes and cytoskeletal polymers, such as actin filaments and microtubules thought to be important for locomotion.

We are also studying the role of the actin cytoskeleton and myosins in the synaptic vesicle recycling.

The third projects focuses on developing new techniques to guide growing neurons in cell culture. We have developed a new method of forming substrate patterns that can be produced after cells have been plated. This allows us to change the direction of growth interactively and form simple neuronal circuits.

Selected Publications:

Turney SG, Ahmed M, Chandrasekar I, Wysolmerski RB, Goeckeler ZM, Rioux RM, Whitesides GM, Bridgman PC. (2016) Nerve growth factor stimulates axon outgrowth through negative regulation of growth cone actomyosin restraint of microtubule advance. Mol. Biol. Cell 2016 27:3 500-517; doi:10.1091/mbc.E15-09-0636

Chandrasekar I, Goeckeler ZM,.Turney SG, Wang P, Robert B. Wysolmerski RB, Adelstein RS, Bridgman PC. (2014) Nonmuscle Myosin II is a Critical Regulator of Clathrin Mediated Endocytosis. Traffic. 2014 Apr;15:418-32. doi: 10.1111/tra.12152. Epub 2014 Feb 12. PMID:24443954

Chandrasekar I, Huettner JE, Turney SG, Bridgman PC. (2013) Myosin II Regulates Activity Dependent Compensatory Endocytosis at Central Synapses. J Neurosci. 2013 Oct 9;33:16131-45. PMID:24107946

JA, Bridgman PC. Disruption of the cytoskeleton during Semaphorin 3A induced growth cone collapse correlates with differences in actin organization and associated binding proteins. Dev. Neurobiol 2009 69:633-646.

Brown JA, Wysolmerski RB, Bridgman PC. Dorsal root ganglion neurons react to semaphorin 3A application through a biphasic response that requires multiple myosin II isoforms. Mol Biol Cell 2009 20:1167-1179.

Turney SG, Bridgman PC. Laminin stimulates and guides axonal outgrowth via growth cone myosin II activity. Nature Neurosci 2005 8:717-719.

Brown ME, Bridgman PC. Retrograde flow rate is increased in growth cones from myosin IIB knockout mice. J Cell Sci 2003 116:1087-1094.

Last Updated: 6/9/2017 11:51:36 AM

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