N. Gautam, Ph.D.

Professor
Anesthesiology
Genetics

Molecular Cell Biology Program
Neurosciences Program
Biochemistry, Biophysics, and Structural Biology Program

  • 314-362-8568

  • 314-362-8526

  • 314-362-8571

  • 8054

  • 5548 Clinical Sciences Research Building

  • http://elysium.wustl.edu/gautam

  • Subcellular optogenetics, light, imaging, signaling, GPCRs, migration, differentiation, contractility, secretion

  • Optogenetic Control of Signaling and Cell Behavior

Research Abstract:

Our goal is to develop and deploy optogenetic approaches that mimic molecular and mechanical stimuli that a cell encounters at the subcellular level and direct important cell behaviors such as migration, cell division and differentiation. By controlling cellular behaviors with light and simultaneously measuring the cellular and molecular responses using imaging methods we identify the mechanisms that govern these behaviors. By regulating cell behaviors at will and monitoring dynamic responses in real time we are able to address questions that have been difficult to address using existing methods.

Selected Publications:

O`Neill PR, Castillo-Badillo JA, Meshik X, Kalyanaraman V, Melgarejo K, Gautam N. (2018) Membrane Flow Drives an Adhesion-Independent Amoeboid Cell Migration Mode. Dev Cell. 46(1):9-22.

George R.R. Bell, Sean R. Collins (2018) “Rho”ing a Cellular Boat with Rearward Membrane Flow Dev Cell. 46, (1)1-3. Article highlighting paper above.

O`Neill PR, Kalyanaraman V, Gautam N. (2016) Subcellular optogenetic activation of Cdc42 controls local and distal signaling to drive immune cell migration. Mol Biol Cell. 1;27(9):1442-50. PMC4850032

Karunarathne WK, O`Neill PR & Gautam N (2015) Subcellular optogenetics - controlling signaling and single-cell behavior. J Cell Sci 128:15-25. PMC4282045

O’Neill, P.R., & Gautam, N. (2014) Subcellular optogenetic inhibition of G proteins generates signaling gradients and cell migration. Mol Biol Cell 25:2305-14. PMC 24920824

Giri, L., Patel, A.K., Karunarathne, W.K.A., Kalyanaraman, V., Venkatesh, K.V., & Gautam, N. (2014) A G Protein Subunit Translocation Embedded Network Motif Underlies GPCR Regulation of Calcium Oscillations. Biophys J 107:242-54. PMC4119271

Karunarathne, W.K., Giri, L., Patel, A.K., Venkatesh, K.V., & Gautam, N. (2013) Optical control demonstrates switch-like PIP3 dynamics underlying the initiation of immune cell migration. Proc Natl Acad Sci USA. 110(17):E1575-83. PMC3637758

Karunarathne, W.K., Giri, L., Kalyanaraman, V., & Gautam, N. (2013) Optically triggering spatiotemporally confined GPCR activity in a cell and programming neurite initiation and extension. Proc Natl Acad Sci USA 110(17):E1565-74. PMC3637763

O`Neill, P.R., Karunarathne, W.K., Kalyanaraman, V., Silvius, J.R., & Gautam, N. (2012) G-protein signaling leverages subunit-dependent membrane affinity to differentially control βγ translocation to intracellular membranes. Proc Natl Acad Sci USA 109(51):E3568-77. PMC3529095

Saini, D.K., Karunarathne, W.K., Angaswamy, N., Saini, D., Cho, J.H., Kalyanaraman, V., & Gautam, N. (2010) Regulation of Golgi structure and secretion by receptor-induced G protein betagamma complex translocation. Proc Natl Acad Sci USA 107: 11417-11422. PMC2895111

Last Updated: 7/11/2018 6:12:30 PM

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