Paul W. Hruz, Ph.D., M.D.

Associate Professor
Pediatrics
Endocrinology/Metabolism
Cell Biology and Physiology

Molecular Cell Biology Program

  • 314-286-2797

  • 314-286-2794

  • 314-286-2892

  • 8208

  • 3108 McDonnell Pediatric Research Building

  • hruz_p@wustl.edu

  • http://peds.wustl.edu/faculty/Hruz_Paul_W/

  • protein structure, glucose transport, diabetes, AIDS, HIV therapy, membrane protein, structural biology, heart, affinity labeling, drug design

  • Structural Biology and Regulation of Glucose Transport; Molecular Mechanisms Leading to Insulin Resistance

Research Abstract:

Our research efforts are directed toward understanding facilitative glucose transport as it relates to normal and disordered glucose homeostasis. A primary effort in our laboratory is the determination of structure/function relationships within the facilitative glucose transport proteins (GLUTs). We are using state of the art chemical and biophysical approaches to the determination of tertiary and quaternary structures of the human erythrocyte glucose transporter GLUT1 and the insulin responsive glucose transporter GLUT4.

In addition, we are also investigating the molecular mechanisms responsible for altered glucose homeostasis during highly active antiretroviral therapy (HAART). Currently we are studying the acute effects of HIV protease inhibitors on peripheral glucose disposal, hepatic glucose production, and insulin secretion using both in vitro and in vivo model systems.

Selected Publications:

Hertel J, Struthers H, Baird Horj C, Hruz, PW. A Structural basis for the acute effects of HIV protease inhibitors on GLUT4 intrinsic activity. J Biol Chem 2004 279(53):55147-55152.

Koster JC, Remidi M, Qiu H, Nichols C, Hruz PW. HIV protease inhibitors acutely impair glucose-stimulated insulin release. Diabetes 2003 52(7):1695-1700.

Hruz PW, Murata H, Qiu H, Mueckler M. Indinavir induces acute and reversible peripheral insulin resistance in rats. Diabetes 2002 51(4):937-942.

Murata H, Hruz PW, Mueckler M. The mechanism of insulin resistance caused by HIV protease inhibitor therapy. J Biol Chem 2000 275:20251-20254.

Hruz PW, Mueckler M. Cysteine-scanning mutagenesis of transmembrane segment 7 of the GLUT1 glucose transporter. J Biol Chem 1999 274:36176-36181.

Last Updated: 2/22/2012 8:00:55 AM

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