Eric A. Galburt, Ph.D.

Associate Professor
Biochemistry and Molecular Biophysics

Biochemistry, Biophysics, and Structural Biology Program

  • 314-362-5201

  • 314-362-4821

  • 8231

  • 660 South Euclid Avenue

  • egalburt@biochem.wustl.edu

  • http://galburtlab.wustl.edu/

  • biophysics, kinetics, optical and magnetic trapping, transcription, fluorescence

  • Biophysical studies of transcription initiation in Eukaryotes and Mycobacterial tuberculosis

Research Abstract:

Work in my laboratory combines biochemical and biophysical techniques to shed light on the detailed interactions between macromolecules that are central to the biology of all organisms. In particular we use single-molecule techniques such as optical and magnetic trapping in combination with fluorescent methods to understand molecular mechanisms leading to transcription and its regulation. Current projects in the lab include open complex formation by the Eukaryotic pre-initiation complex and mechanisms of mycobacterial transcription regulation.

Selected Publications:

Tomko E.J., Fishburn J., Hahn S., Galburt E.A. (2017) TFIIH generates a six-base-pair open complex during RNAP II transcription initiation and start-site scanning Nature Structural and Molecular Biology

Galburt E.A. and Rammohan J. (2016) A kinetic signature for Parallel Pathways: Conformational Selection and Induced Fit Biochemistry

Rammohan J., Ruiz Manzano A., Garner A.L., Prusa, J., Stallings, C.L., Galburt, E.A. (2016) Cooperative stabilization of Mycobacterium tuberculosis rrnAP3 promoter open complexes by RbpA and CarD. Nucleic Acids Research

Fishburn, J., Galburt, E.A., Hahn, S. (2016) Transcription Start Site Scanning and the Requirement for ATP During Transcription Initiation by RNA Polymerase II. J. Biol. Chem.

Fishburn, J., Tomko, E.J., Galburt, E.A., Hahn, S. (2015) Double-stranded DNA translocase activity of TFIIH and the mechanism of RNAP Polymerase II Open Complex formation. PNAS. 112(13):3961-6.

Rammohan, J. Ruiz Manzano, A., Garner, A.L., Stallings, C.L., Galburt, E.A. (2015) CarD stabilizes mycobacterial open complexes via a two-tiered kinetic mechanism. Nucleic Acids Res. 43(6):3272-85.

Galburt, E.A., Tomko, E.J., Stump, W.T. and Ruiz Manzano, A. (2014) Force-dependent melting of supercoiled DNA at thermophilic temperatures. Biophys Chem. 187-188:23-28.

Galburt EA, Parrondo, Grill SW.(2011) RNA polymerase pushing. Biophysical Chemistry.157(1-3):43-47.

Depken M, Galburt EA, Grill SW.(2009) The origin of short transcriptional pauses. Biophysical Journal. 1896(6):2189-93. PMCID: PMC2717293

Galburt EA, Grill SW, Wiedmann A, Lubkowska L, Choy J, Nogales E, Kashlev M and Bustamante CJ. (2007) Backtracking determines the force sensitivity of RNAP II in a factor-dependent manner. Nature. 446(7137):820-3.

Last Updated: 7/27/2018 11:28:15 AM

Magnetic tweezers assay for measuring DNA unwinding during transcription initiation.
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