Eric A. Galburt, Ph.D.

Associate Professor
Biochemistry and Molecular Biophysics

Biochemistry, Biophysics, and Structural Biology Program

  • 314-362-5201

  • 314-362-4821

  • 8231

  • 660 South Euclid Avenue



  • biophysics, molecular motors, optical trapping, transcription

  • Transcription; Single Molecule; Optical Trapping; Magnetic Trapping; Biophyiscs

Research Abstract:

Work in my laboratory combines biochemical and biophysical techniques to shed light on the detailed interactions between macromolecules that are central to the biology of all organisms. In particular we use single-molecule techniques such as optical and magnetic trapping in combination with fluorescent methods to understand molecular mechanisms leading to transcription and its regulation. Current projects in the lab include open complex formation by the Eukaryotic pre-initiation complex and mechanisms of mycobacterial transcription regulation.

Selected Publications:

Rammohan J., Ruiz Manzano A., Garner A.L., Prusa, J., Stallings, C.L., Galburt, E.A. (2016) Cooperative stabilization of Mycobacterium tuberculosis rrnAP3 promoter open complexes by RbpA and CarD. Nucleic Acids Res.

Fishburn, J., Galburt, E.A., Hahn, S. (2016) Transcription Start Site Scanning and the Requirement for ATP During Transcription Initiation by RNA Polymerase II. J. Biol. Chem.

Lisica, A., Engel, C. Jahnel, M., Roldán, É., Galburt, E.A., Cramer, P., Grill, S.W. (2016) Mechanisms of backtrack recovery by RNA polymerases I and II. PNAS.

Fishburn, J., Tomko, E.J., Galburt, E.A., Hahn, S. (2015) Double-stranded DNA translocase activity of TFIIH and the mechanism of RNAP Polymerase II Open Complex formation. PNAS. 112(13):3961-6.

Rammohan, J. Ruiz Manzano, A., Garner, A.L., Stallings, C.L., Galburt, E.A. (2015) CarD stabilizes mycobacterial open complexes via a two-tiered kinetic mechanism. Nucleic Acids Res. 43(6):3272-85.

Garner,A.L., Weiss, L.A., Manzano, A.R., Galburt, E.A. and Stallings, C.L. (2014) CarD Integrates Three Functional Modules to Promote Efficient Transcription, Antibiotic Tolerance, and Pathogenesis in Mycobacteria. Mol Microbiol. 93(4):682-97.

Galburt, E.A., Tomko, E.J., Stump, W.T. and Ruiz Manzano, A. (2014) Force-dependent melting of supercoiled DNA at thermophilic temperatures. Biophys Chem. 187-188:23-28.

Galburt EA, Parrondo, Grill SW.(2011) RNA polymerase pushing. Biophysical Chemistry.157(1-3):43-47.

Depken M, Galburt EA, Grill SW.(2009) The origin of short transcriptional pauses. Biophysical Journal. 1896(6):2189-93. PMCID: PMC2717293

Galburt EA, Grill SW, Wiedmann A, Lubkowska L, Choy J, Nogales E, Kashlev M and Bustamante CJ. (2007) Backtracking determines the force sensitivity of RNAP II in a factor-dependent manner. Nature. 446(7137):820-3.

Last Updated: 8/13/2016 1:12:52 PM

Transcriptional regulators CarD and RbpA bound to RNA polymerase initiation complex.
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