Mikhail Y. Berezin, Ph.D.
Computational and Molecular Biophysics Program
biophysics, cancer, electrochemistry, fluorescence, nanotechnology, imaging and spectroscopy instrumentation
Investigation of the optical contrast agents and spectrscopy for medical imaging and clinical treatment
My research interest lies in the investigation and application of molecular excited states and their reactions for medical imaging and clinical treatment. Excited states are the cornerstone of a variety of chemical, physical, and biological phenomena. The ability to probe, investigate, and control excited states is one of the largest achievements of modern science. The lab focuses on the development of novel optically active probes ranging from small molecules to nanoparticles, and the development of optical instrumentation for spectroscopy and imaging and their applications in medicine. The central obstacle in optical imaging of biological tissue is the transport of photons through tissue. Photon penetration into living tissue is highly dependent on the absorption and scattering properties of tissue components and significantly increases above 650 nm. Hence, the near-infrared (NIR) region of the spectrum (650-900 nm) has been widely explored, and a number of contrast probes active in this region have been developed. Currently we are developing optically active in NIR spectral range activatable fluorescence probes and nanoparticles for diagnostics and treatment. Although NIR range is well suitable for tissue penetration, this region still suffers from large scattering and residual absorbance from endogenous chromophores. As a result, the depth penetration of NIR based optical imaging is limited to several millimeters severely weakening its applicability for deeper tissue imaging. To minimize the problem with scattering and absorption while increasing the penetration depth we propose to use utilized the extended NIR (exNIR) range from 1000-1400 nm. This region is mostly free from water and other endogenous chromophores, leaving this optical window absorption transparent with virtually no autofluorescence. We are currently synthesizing an array of exNIR fluorescent dyes with high quantum yield for in vivo imaging.
GustafsonTP, YanP, Newton P, Hunter DA, Achilefu S, Akers WJ, Mackinnon SE, Johnson PJ and Berezin MY. A NIR Dye for Development of Peripheral Nerve Targeted Probes. MedChemCommun 2012 (In Print).
Gustafson TP, Cao Q, Achilefu S, Berezin MY. Defining a Polymethine Dye for Fluorescence Anisotropy Applications in the NIRSpectralRange. ChemPhysChem 2012 (In Print).
Guo K, Achilefu S, Berezin MY. Dating Bloodstains with Fluorescence Lifetime Measurements. Chemistry Eur. J. 2012 (DOI: 10.1002/chem.201102935).
Berezin MY, Zhan C, Lee H, Joo C, Akers WJ, Yazdanfar S, Achilefu S. Two-photon optical properties of near-infrared dyes at 1.55 μm excitation. J. Phys. Chem. B. 2011 115(39):11530-5.
Akers W, Kim C, Berezin M, Guo K, Fuhrhop R, Lanza G, Fischer G, Daltrozzo E, Zumbusch A, Cai X, Wang L, Achilefu S. Non-invasive Photoacoustic and Fluorescence Sentinel Lymph Node Identification using Dye-loaded Perfluorocarbon Nanoparticles. ACS Nano 2011 5(1): 173-82.
Berezin M, Guo K, Akers W, Livingston J, Solomon M, Lee H, Liang K, Agee A, Achilefu S. Rational Approach to Select Small Peptide Molecular Probes Labeled with Fluorescent Cyanine Labels Dyes for in vivo Optical Imaging. Biochemistry 2011 50(13):2691-700).
Yazdanfar S, Joo C, Zhan C, Berezin MY, Akers W, Ye Y, Achilefu S. Multiphoton microscopy of near infrared contrast agents, J. Biomed. Optics 2010 15: 030505.
Berezin MY, Achilefu S. Fluorescence Lifetime Measurements and Biological Imaging, Chem. Rev. 2010 110(5): 2641-2684.
Akers W, Zhang Z, Fuhrhop R, Berezin MY, Ye Y, Agee A, Guo K, Lanza G, Achilefu S. Targeting of a3-Integrins Expressed on Tumor Tissue and Neovasculature Using Fluorescent Small Molecules and Nanoparticles. Nanomedicine 2010 5(5): 715-26
Berezin MY, Akers W, Guo K, Fischer GM, Daltrozzo E, Zumbusch A, Achilefu S. Long Fluorescence Lifetime Molecular Probes Based on Near Infrared Pyrrolopyrrole Cyanine Fluorophores for in vivo imaging, Biophys. J. 2009 97: L22-L24.
Berezin MY, Lee H, Akers W, Achilefu S. Near Infrared Dyes as Lifetime Solvatochromic Probes for Micropolarity Measurements of Biological Systems. Biophys. J. 2007 93: 2892-2899.
Last Updated: 8/9/2013 2:52:55 PM