Jonathan R. Silva, Ph.D.
Assistant Professor
Biomedical Engineering
Biochemistry, Biophysics, and Structural Biology Program
Molecular Cell Biology Program
Computational and Systems Biology Program
314-935-8837
314-935-9553
314-935-7448
1097
Whitaker Hall 235
jonsilva@wustl.edu
http://silvalab.bme.wustl.edu/
Cardiac, Bioelectricity, Ion Channel, Sodium Channel, Action Potential, Modeling, Electrophysiology, Voltage Clamp Fluorometry
Predicting how changes in ion channel nano-conformations affect cell and organ electrophysiology
Research Abstract:
Neuronal and myocyte bioelectricity arises from the atomic interactions of ion channels. While the atoms and molecules that form channels interact at nanometer and nanosecond time-scales, heart beats and neuronal signals travel over centimeters and seconds. Bridging these time and length scales is a primary barrier to developing useful small molecules therapies. To gain a multiscale understanding, we use computational models that are based on data we collect with cutting-edge biophysics methods.
Selected Publications:
Varga, Z., Zhu, W., Schubert, A. R., Pardieck, J. L., Krumholz, A., Hsu, E. J., ... & Silva, J. R. (2015). Direct Measurement of Cardiac Na+ Channel Conformations Reveals Molecular Pathologies of Inherited Mutations. Circulation: Arrhythmia and Electrophysiology, CIRCEP-115. PMID: 26283144
Silva, J.R. & Rudy, Y. (2010). Multi-scale electrophysiology modeling: from atom to organ. Journal of General Physiology 135 (6), 575. PMID: 20513759.
Silva, J.R., et al (2009). A multiscale model linking ion-channel molecular dynamics and electrostatics to the cardiac action potential. Proceedings of the National Academy of Sciences, 106(27), 11102-11106. PMID: 19549851.
Last Updated: 9/22/2015 10:34:25 AM
Schematic of the voltage clamp fluorometry protocol.