Eli Roberson, Ph.D.

Assistant Professor
Internal Medicine

Human and Statistical Genetics Program
Molecular Genetics and Genomics Program
Computational and Systems Biology Program

  • 314 362-9736

  • 314-454-3634

  • 314 362-9257

  • 8045

  • CSRB 10008

  • eroberson@WUSTL.EDU

  • http://www.robersonlab.org

  • human genetics; autoimmune disease; bioinformatics; sequencing; biomarkers

  • Human genetics and RNA-Seq of inflammatory and autoimmune disease, particularly those with an overt skin phenotype

Research Abstract:

Our lab is primarily focused on the human genetics and molecular biology of inflammatory and autoimmune disease, mostly those that affect skin.

We currently study hidradenitis suppurativa, juvenile dermatomyositis, and systemic sclerosis. The goals of our disease research are to 1) identify dysregulated pathways by RNA-Seq of affected tissues, 2) identify disease activity biomarkers from blood samples to stratify disease risk, and 3) discover rare variants influencing disease risk that we can then model in the lab.

For our diseases of interest, we approach the genetic variation question as one of populations and families. We collect families enriched for uncommon diseases to identify rare and novel variants segregating with disease using high-throughput sequencing. We then use genome editing to introduce variants of interest into relevant cell lines to study their effects. Similarly, we bank DNA on as many sporadic cases as we can collect for further study using targeted capture or common variant association.

We collect blood and affected tissue, usually skin biopsies, from patients as well. These tissues are critically important to the direct study of immune dysregulation, pathway identification, and biomarker identification.

The theme running through all of our research is the dissection of human inflammatory / autoimmune disease. Importantly, our approaches combine both traditional wet bench molecular and cell biology with computational and statistical approaches. We design the necessary experiments, develop new methodology as needed, generate data, design software tools
to process the data we generate, and analyze the data using freely available software.

Our lab is therefore a unique incubator with many different possible training focuses.

Selected Publications:

Roberson EDO. Identification of high-efficiency 3’GG gRNA motifs in indexed FASTA files with ngg2. PeerJ Computer Science 2015. 1:e33. PMCID: PMC4750479.

Triebwasser MP†, Roberson EDO†, Yu Y, Schramm EC, Raychaudhuri S, Seddon JM, Atkinson JP. Rare variants in the functional domains of Complement Factor H are associated with age-related macular degeneration. Investigative Ophthalmology and Visual Science 2015: 56(11):6873-6878. PMCID: PMC4627248.

Jordan CT, Cao L, Roberson EDO, Pierson KC, Yang C-F, Joyce CE, Ryan C, Duan S, Helms CA, Liu Y, Chen Y, McBride AA, Hwu W-L, Wu J-Y, Chen Y-T, Menter A, Goldbach-Mansky R, Lowes MA, Bowcock AM. PSORS2 is due to mutations in CARD14. The American Journal of Human Genetics 2012, 90(5): 784-795. PMCID: PMC3376640.

Roberson EDO†, Liu Y†, Ryan C, Joyce CE, Duan S, Cao L, Martin A, Liao W, Menter A, Bowcock AM. A subset of methylated CpG sites differentiate psoratic from normal skin. Journal of Investigative Dermatology 2011: 132(3 Pt 1): 583-592. PMCID: PMC3568942.

Harbour JW, Onken MD, Roberson EDO, Duan S, Cao L, Worley LA, Council ML, Matatall KA, Helms C, Bowcock AM. Frequent mutations of BAP1 in metastasizing uveal melanomas. Science 2010: 330(6009): 1410-1413. PMCID: PMC3087380.

Last Updated: 9/1/2016 9:16:57 AM

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