Michael J. Greenberg, Ph.D.

Assistant Professor
Biochemistry and Molecular Biophysics

Biochemistry, Biophysics, and Structural Biology Program
Molecular Cell Biology Program
Developmental, Regenerative and Stem Cell Biology Program

  • 314 362-8670

  • 314 362-7434

  • 314 362-7183

  • 8231

  • 253 McDonnell Medical Sciences Building

  • greenberg@biochem.wustl.edu

  • http://glab.biochem.wustl.edu/

  • myosin, heart disease, single molecule, optical trapping, muscle, cardiac, stem cells, tissue engineering, cytoskeletal molecular motors, mechanobiology, computational modeling

  • Molecular motors and the mechanobiology of heart disease

Research Abstract:

Our lab is interested in understanding molecular motors and their role in mechanobiology. Currently, the lab is studying mutations in contractile proteins that cause familial cardiomyopathies, the leading cause of sudden cardiac death in people under 30 years old. The lab uses an array of biochemical, biophysical, cell biological, and engineering techniques to decipher how these mutations affect the mechanobiology of heart contraction across scales from the level of single molecules to the level of engineered tissues. We then use computational modeling to link these scales. For our molecular studies, we have developed an optical trap with fast feedback to study the contractility of single molecules. For our cellular and tissue-level studies, we have used genetic and tissue engineering to develop human stem cell-based models of the disease. Insights gleaned from our studies will guide efforts to develop novel therapies.

Selected Publications:

Greenberg MJ, Daily N, Wang A, Conway MK, Wakatsuki T. (2018). Genetic and tissue engineering approaches to modeling the mechanics of human heart failure for drug discovery. In press at Trends in Cardiovascular Medicine.

Woody MS, Greenberg MJ, Barua B, Winkelmann DA, Goldman YE, Ostap EM. (2018). Positive cardiac inotrope, omecamtiv mecarbil, activates muscle despite suppressing the myosin working stroke. In press at Nature Communications.

Greenberg MJ, Shuman H, Ostap EM. (2017). Measuring the Kinetic and Mechanical
Properties of Non-processive Myosins Using Optical Tweezers. Methods in Molecular Biology, 1486:483-509. PMID: 27844441.

Woody MS, Lewis JH, Greenberg MJ, Goldman YE, Ostap EM. (2016). MEMLET: An Easy-to-Use Tool for Data Fitting and Model Comparison Using Maximum-Likelihood Estimation. Biophysical Journal, 111(2):273-82. PMCID: PMC4968482.

Greenberg MJ, Arpağ G, Tüzel E, Ostap EM. A Perspective on the Role of Myosins as Mechanosensors. (2016). Biophysical Journal, 110(12):2568-76. PMCID: PMC4919425.

Greenberg, M.J., Lin, T., Shuman, H. and Ostap, E.M. (2015). Mechanochemical tuning of myosin-I by the N-terminal region. Proceedings of the National Academy of Sciences, 112:E3337-E3344. PMCID: PMC4491760.

Greenberg, M.J., Shuman, H. and Ostap, E.M. (2014). Inherent force-dependent properties of β-cardiac myosin contribute to the force-velocity relationship of cardiac muscle. Biophysical Journal, 107:L41-4. PMCID: PMC4269798.

Shuman, H., Greenberg, M.J., Zwolak, A., Lin, T., Sindelar, C.V., Dominguez, R. and Ostap, E.M. (2014). A vertebrate myosin-I structure reveals unique insights into myosin mechanochemical tuning. Proceedings of the National Academy of Sciences, 111:2116-2121. PMCID: PMC3926069.

Greenberg, M.J., Lin, T., Goldman, Y.E., Shuman, H., Ostap, E.M. (2012). Myosin IC generates power over a range of loads via a new tension-sensing mechanism. Proceedings of the National Academy of Sciences, 109(37):E2433-40. PMCID: PMC3443183.

Last Updated: 8/14/2018 2:12:32 PM

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