Michael J. Greenberg, Ph.D.

Assistant Professor
Biochemistry and Molecular Biophysics

Biochemistry, Biophysics, and Structural Biology Program
Molecular Cell Biology Program
Developmental, Regenerative and Stem Cell Biology Program

  • 314 362-8670

  • 314 362-7434

  • 314 362-7183

  • 8231

  • 253 McDonnell Medical Sciences Building

  • greenberg@biochem.wustl.edu

  • http://glab.biochem.wustl.edu/

  • myosin, actin, single molecule, optical trapping, muscle, cardiac, stem cells, engineered tissue, molecular motors, mechanosensing, cytoskeleton

  • Cytoskeletal molecular motors in health and disease. We are currently studying the effects of mutations that cause heart disease.

Research Abstract:

The Greenberg lab focuses on how cytoskeletal motors function in both health and disease. Currently, the lab is studying mutations that cause familial cardiomyopathies, the leading cause of sudden cardiac death in people under 30 years old. The lab uses an array of biochemical, biophysical, and cell biological techniques to decipher how these mutations affect heart contraction from the level of single molecules to the level of engineered tissues. We are also interested in developing human stem cell-based models of the disease. Insights into the disease pathogenesis will guide efforts to develop novel therapies.

Selected Publications:

Greenberg MJ, Shuman H, Ostap EM. (2017). Measuring the Kinetic and Mechanical
Properties of Non-processive Myosins Using Optical Tweezers. Methods in Molecular Biology, 1486:483-509. PMID: 27844441.

Woody MS, Lewis JH, Greenberg MJ, Goldman YE, Ostap EM. (2016). MEMLET: An Easy-to-Use Tool for Data Fitting and Model Comparison Using Maximum-Likelihood Estimation. Biophysical Journal, 111(2):273-82. PMCID: PMC4968482.

Greenberg MJ, Arpağ G, Tüzel E, Ostap EM. A Perspective on the Role of Myosins as Mechanosensors. (2016). Biophysical Journal, 110(12):2568-76. PMCID: PMC4919425.

Kee, A.J., Yang, L., Lucas, C.A., Greenberg, M.J., Martel, N., Leong, G.M., Hughes, W.E., Cooney, G.J., James, D.E., Ostap, E.M., Han, W., Gunning, P.W. and Hardeman, E.C. (2015). An actin filament population defined by the tropomyosin Tpm3.1 regulates glucose uptake. Traffic, 16:691-711. PMCID: PMC4945106.

Greenberg, M.J., Lin, T., Shuman, H. and Ostap, E.M. (2015). Mechanochemical tuning of myosin-I by the N-terminal region. Proceedings of the National Academy of Sciences, 112:E3337-E3344. PMCID: PMC4491760.

Greenberg, M.J., Shuman, H. and Ostap, E.M. (2014). Inherent force-dependent properties of β-cardiac myosin contribute to the force-velocity relationship of cardiac muscle. Biophysical Journal, 107:L41-4. PMCID: PMC4269798.

Shuman, H., Greenberg, M.J., Zwolak, A., Lin, T., Sindelar, C.V., Dominguez, R. and Ostap, E.M. (2014). A vertebrate myosin-I structure reveals unique insights into myosin mechanochemical tuning. Proceedings of the National Academy of Sciences, 111:2116-2121. PMCID: PMC3926069.

Greenberg, M.J. and Ostap, E.M. (2013). Regulation and control of myosin-I by the motor and light chain-binding domains. Trends in Cell Biology, 23:81-9. PMCID: PMC3558615.

Greenberg, M.J., Lin, T., Goldman, Y.E., Shuman, H., Ostap, E.M. (2012). Myosin IC generates power over a range of loads via a new tension-sensing mechanism. Proceedings of the National Academy of Sciences, 109(37):E2433-40. PMCID: PMC3443183.

Last Updated: 8/23/2017 9:41:26 AM

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