Matthew D. Lew, Ph.D.

Assistant Professor
Electrical & Systems Engineering

Biochemistry, Biophysics, and Structural Biology Program

  • 314-935-6790

  • 314-935-9070

  • 314-935-7500

  • Green Hall 2158



  • 3D super-resolution microscopy, single-molecule spectroscopy, imaging molecular orientation, computational optics

  • Developing novel optical tools for biology and medicine

Research Abstract:

The Lew Lab is a group of passionate scientists and engineers to build technologies that will enable discoveries of how life works at the nanoscale. We are a team of inventors and problem solvers working at the intersection of science and technology, leveraging innovations in modern optics, image processing, chemistry, and biotechnology to create imaging systems with unprecedented performance.

Current Projects
New Imaging Technologies:
- Tri-Spot Point Spread Function
- Robust Statistical Estimation (RoSE) algorithm
- nanoscale protein aggregation in Alzheimer’s disease
- structure and organization of lipid membranes
- imaging extracellular electron transport in bacteria

Please see our website at for more information.

Selected Publications:

1. Zhang O, Lu J, Ding T, Lew MD. (2018). Imaging the three-dimensional orientation and rotational mobility of fluorescent emitters using the Tri-spot point spread function. Applied physics letters, 113(3):031103. PMID: 30057423, PMCID: PMC6050162

2. Spehar K, Ding T, Sun Y, Kedia N, Lu J, Nahass GR, Lew MD, Bieschke J. (2018). Super-resolution Imaging of Amyloid Structures over Extended Times by Using Transient Binding of Single Thioflavin  T Molecules. Chembiochem. PMID: 29953718

3. Liba O, Lew MD, SoRelle ED, Dutta R, Sen D, Moshfeghi DM, Chu S, de la Zerda A. (2017). Speckle-modulating optical coherence tomography in living mice and humans. Nature communications, 8:15845. PMID: 28632205, PMCID: PMC5481831

4. Lew MD, Moerner WE. (2014). Azimuthal polarization filtering for accurate, precise, and robust single-molecule localization microscopy. Nano letters, 14(11):6407-13. PMID: 25272093, PMCID: PMC4245985

5. Backlund MP, Lew MD, Backer AS, Sahl SJ, Moerner WE. (2014). The role of molecular dipole orientation in single-molecule fluorescence microscopy and implications for super-resolution imaging. Chemphyschem, 15(4):587-99. PMID: 24382708, PMCID: PMC3992256

Last Updated: 8/17/2018 1:43:00 PM

Recipient of 2017 NSF CAREER Award
Back To Top

Follow us: