Research Abstract:
The long-term goal of our research is to understand the molecular basis of cell motility, including the role of motility in human disease, especially cancer. How do actin, microtubules and their motors control the shape and movement of cells? How are these elements of the cytoskeleton regulated by signals from the cell cycle machinery and from outside the cell? How is cell cycle progression regulated by checkpoints based on the cytoskeleton? We study these questions in vertebrates and yeast, using the strengths of each system. We utilize a variety of technical approaches encompassing genetics, biochemistry and cell biology.
Current projects in the lab include the following: 1) Actin assembly and membrane dynamics in animal cells: We are studying how actin-binding proteins regulated by signals from cancer-causing viruses and growth factors direct the polymerization of actin, which causes cells to move and change shape. 2) Actin in yeast: In yeast, actin is the basis for endocytosis and polarized growth. We are interested in the molecular mechanisms that underlie these processes. 3) Mitosis in yeast: We are interested in how microtubules and their motors function in mitosis, in particular how microtubules interact with the cell cortex to position the mitotic spindle. 4) Cell cycle control: In yeast, we discovered a new cell cycle checkpoint that connects mitosis and cell division, with a connection to human cancer. We are investigating the molecular basis of this checkpoint.
Selected Publications:
Kim K, McCully ME, Bhattacharya N, Butler B, Sept D, Cooper JA. Structure/Function Analysis of the Interaction of Phosphatidylinositol 4,5-Bisphosphate with Actin-capping Protein: Implications for How Capping Protein Binds the Actin Filament. J Biol Chem 2007 282:5871-5879.
Nelson SA, Cooper JA. A Novel Pathway that Coordinates Mitotic Exit with Spindle Position. Mol Biol Cell 2007 18:3440-3450.
Tehrani S, Tomasevic N, Weed S, Sakowicz R, Cooper JA. Src phosphorylation of cortactin enhances actin assembly. Proc Natl Acad Sci USA 2007 104:11933-11938.
Bhattacharya N, Ghosh S, Sept D, Cooper JA. Binding of Myotrophin/V-1 to Actin-capping Protein: Implications for How Capping Protein binds to the Filament Barbed End. J Biol Chem 2006 281:31021-31030.
Tehrani S, Faccio R, Chandrasekar I, Ross FP, Cooper JA. Cortactin Has an Essential and Specific Role in Osteoclast Actin Assembly. Mol Biol Cell 2006 17:2882-2895.
Last Updated: 09/05/2007 |