• Tsinghua University (2015)

  • Molecular Genetics and Genomics

  • Ting Wang, Ph.D.

  • Understand the dynamics of cell-type composition changes during regenerative process

  • yiran@wustl.edu

Research

Blastema formation is the key process in appendage regeneration. The underlying mechanism that support this process may help us understand what we missed for a concise regeneration. In the zebrafish caudal fin model, independent observations show that the blastema is contributed by both dedifferentiation of adult lineage cells and proliferation of resident progenitors. However, due to resolution limitations, the path of cell-state transition for these cells have not been clearly revealed. The two cell sources may share part of their path during regeneration, with adult lineage cells contributing to the progenitor population. To determine whether adult lineage cells join regeneration via dedifferentiating into a progenitor-like phase, I propose to track the cell-state of blastemal cells during regeneration by single-cell-RNA sequencing. Measurement and comparison of transcriptome in adult lineage cells and the progenitors at single-cell resolution may shed light on how each lineage respond and react during regeneration.

Graduate Publications:

Lee HJ, Hou Y, Maeng JH, Shah NM, Chen Y, Lawson HA, Yang H, Yue F, Wang T. 2022 Epigenomic analysis reveals prevalent contribution of transposable elements to cis-regulatory elements, tissue-specific expression, and alternative promoters in zebrafish. Genome Res, 32(7):1424-36.

Jang HS, Chen Y, Ge J, Wilkening AN, Hou Y, Lee HJ, Choi YR, Lowdon RF, Xing X, Li D, Kaufman CK, Johnson SL, Wang T. 2021 Epigenetic dynamics shaping melanophore and iridophore cell fate in zebrafish. Genome Biol, 22(1):282.

Lee HJ, Hou Y, Chen Y, Dailey ZZ, Riddihough A, Jang HS, Wang T, Johnson SL. 2020 Regenerating zebrafish fin epigenome is characterized by stable lineage-specific DNA methylation and dynamic chromatin accessibility. Genome Biol, 21(1):52.

Hou Y, Lee HJ, Chen Y, Ge J, Osman FOI, McAdow AR, Mokalled MH, Johnson SL, Zhao G, Wang T. 2020 Cellular diversity of the regenerating caudal fin. Sci Adv, 6(33):eaba2084.

Last Updated: 3/12/2018 1:07:04 PM

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