• University of South Florida (2018)

  • Plant and Microbial Biosciences

  • Petra Anne Levin, Ph.D.

  • Immunofluorescent microscopy of FtsZ C-Terminal Linker Varients in Bacillus subtilis

  • jlbullock@wustl.edu

Research

First Rotation: Levin Lab

Immunofluorescent microscopy of FtsZ C-Terminal Linker Varients in Bacillus subtilis

FtsZ is an essential cell division protein that forms ring like structures mid-cell and acts as a scaffold for cell division machinery. FtsZ is a tubulin like protein that consists of a GTP binding domain, an intrinsically disordered linker domain referred to as the C terminal Linker (CTL), and a grappling hook peptide. The CTL is not conserved in primary sequence or length, but is necessary for cell division. Kappa, a measure of charge distribution, is highly conserved among CTLs. The goal of this project was to determine the extent to which Kappa plays a tole in CTL function and therefore FtsZ function. During my rotation I used immunofluorescent microscopy to visualize the effects of Kappa variance on FtsZ ring formation.

Second Rotation: Kutluay Lab

In progress

Last Updated: 9/9/2019 3:59:37 PM

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