N. Gautam, Ph.D.


Molecular Cell Biology Program
Neurosciences Program
Biochemistry, Biophysics, and Structural Biology Program

  • 314-362-8568

  • 314-362-8526

  • 314-362-8571

  • 5548 Clinical Sciences Research Building

  • Subcellular optogenetics, light, imaging, signaling, cell migration, cell shape, cell division

  • Optogenetic Control of Signaling and Single Cell Behavior

Research Abstract:

Our goal is to develop and deploy optogenetic methods that mimic molecular and mechanical stimuli that a cell encounters at the subcellular level and optically direct important behaviors such as cell migration, cell division and cell shape. By controlling cellular behaviors with light and simultaneously measuring the cellular and molecular responses using imaging methods we identify the mechanisms that govern these behaviors. By regulating cell behaviors at will and monitoring dynamic responses in real time we are able to address questions that have been difficult to address using existing methods.

Selected Publications:

Castillo-Badillo JA, Gautam N. (2021) Optogenetic Model Reveals Cell Shape Regulation Through FAK and Fascin. J Cell Sci (In press).

Castillo-Badillo JA, Bandi AC, Harlalka S, Gautam N. (2020) SRRF-Stream Imaging of Optogenetically Controlled Furrow Formation Shows Localized and Coordinated Endocytosis and Exocytosis Mediating Membrane Remodeling. ACS Synth Biol 9(4):902-919.

Xenia Meshik, Patrick R O'Neill, N Gautam. (2019) Physical Plasma Membrane Perturbation Using Subcellular Optogenetics Drives Integrin-Activated Cell Migration. ACS Synth Biol. 15;8(3):498-510.

O`Neill PR, Castillo-Badillo JA, Meshik X, Kalyanaraman V, Melgarejo K, Gautam N. (2018) Membrane Flow Drives an Adhesion-Independent Amoeboid Cell Migration Mode. Dev Cell. 46(1):9-22.

O`Neill PR, Kalyanaraman V, Gautam N. (2016) Subcellular optogenetic activation of Cdc42 controls local and distal signaling to drive immune cell migration. Mol Biol Cell. 1;27(9):1442-50.

Karunarathne WK, O`Neill PR & Gautam N (2015) Subcellular optogenetics - controlling signaling and single-cell behavior. J Cell Sci 128:15-25.

O’Neill, P.R., & Gautam, N. (2014) Subcellular optogenetic inhibition of G proteins generates signaling gradients and cell migration. Mol Biol Cell 25:2305-14.

Giri, L., Patel, A.K., Karunarathne, W.K.A., Kalyanaraman, V., Venkatesh, K.V., & Gautam, N. (2014) A G Protein Subunit Translocation Embedded Network Motif Underlies GPCR Regulation of Calcium Oscillations. Biophys J 107:242-54. 

Karunarathne, W.K., Giri, L., Patel, A.K., Venkatesh, K.V., & Gautam, N. (2013) Optical control demonstrates switch-like PIP3 dynamics underlying the initiation of immune cell migration. Proc Natl Acad Sci USA. 110(17):E1575-83.

Karunarathne, W.K., Giri, L., Kalyanaraman, V., & Gautam, N. (2013) Optically triggering spatiotemporally confined GPCR activity in a cell and programming neurite initiation and extension. Proc Natl Acad Sci USA 110(17):E1565-74.

O`Neill, P.R., Karunarathne, W.K., Kalyanaraman, V., Silvius, J.R., & Gautam, N. (2012) G-protein signaling leverages subunit-dependent membrane affinity to differentially control βγ translocation to intracellular membranes. Proc Natl Acad Sci USA 109(51):E3568-77.<o:p></o:p>

Last Updated: 6/9/2021 9:51:33 PM

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