Andrea Soranno, Ph.D.

Assistant Professor
Biochemistry and Molecular Biophysics

Biochemistry, Biophysics, and Structural Biology Program

  • 314-273-1632

  • 2913 South Bldg.

  • soranno@wustl.edu

  • My main research interests are the physical principles and molecular mechanisms determining biomolecular function.

Research Abstract:

My main research interests are the physical principles and molecular mechanisms determining biomolecular
function. A particular focus are intrinsically disordered proteins (IDPs). At variance with the classical structurefunction paradigm that we are familiar with from 50 years of structural biology, IDPs lack a persistent structure but, nevertheless, participate in many biological processes such as transcription, translation and signal
transduction. In my work, I combine fluorescence single-molecule spectroscopy with concepts from polymer
physics to explain fundamental properties of IDPs. I am interested in expanding the scope of single-molecule
and correlation methods to investigate the role of disordered proteins in vivo, especially within RNA granules,
non-membrane bound organelles that play a key-role in the spatial and temporal regulation of RNA, and whose
malfunctioning has been linked to infertilities, cancer, and neurodegenerative diseases. A long-term goal will be
to bridge the gap between detailed molecular understanding and cellular and organismic processes.

Selected Publications:

Integrated view of internal friction in unfolded proteins from single-molecule FRET, contact quenching, theory, and simulations.
Soranno A, Holla A, Dingfelder F, Nettels D, Makarov DE, Schuler B.
Proceedings of the National Academy of Sciences of the United States of America. 2017; 114(10):E1833-E1839.

Consistent View of Polypeptide Chain Expansion in Chemical Denaturants from Multiple Experimental Methods.
Borgia A, Zheng W, Buholzer K, Borgia MB, Schüler A, Hofmann H, Soranno A, Nettels D, Gast K, Grishaev A, Best RB, Schuler B.
Journal of the American Chemical Society. 2016; 138(36):11714-26.

Comprehensive structural and dynamical view of an unfolded protein from the combination of single-molecule FRET, NMR, and SAXS.
Aznauryan M, Delgado L, Soranno A, Nettels D, Huang JR, Labhardt AM, Grzesiek S, Schuler B.
Proceedings of the National Academy of Sciences of the United States of America. 2016; 113(37):E5389-98.

Single-Molecule FRET Spectroscopy and the Polymer Physics of Unfolded and Intrinsically Disordered Proteins.
Schuler B, Soranno A, Hofmann H, Nettels D.
Annual review of biophysics. 2016; 45:207-31.

Transient misfolding dominates multidomain protein folding.
Borgia A, Kemplen KR, Borgia MB, Soranno A, Shammas S, Wunderlich B, Nettels D, Best RB, Clarke J, Schuler B.
Nature communications. 2015; 6:8861.

Single-molecule spectroscopy of protein conformational dynamics in live eukaryotic cells.
König I, Zarrine-Afsar A, Aznauryan M, Soranno A, Wunderlich B, Dingfelder F, Stüber JC, Plückthun A, Nettels D, Schuler B.
Nature methods. 2015; 12(8):773-9.

Last Updated: 8/2/2017 3:31:07 PM

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