Abby M. Green, MD

Assistant Professor
Pediatrics
Infectious Diseases
Pathology and Immunology

Cancer Biology Program
Molecular Cell Biology Program
Molecular Genetics and Genomics Program

Research Abstract:

Overview:
Cancer develops through accumulation of DNA mutations and structural aberrations collectively known as genome instability. Genome damage in adult-onset malignancies can be traced to exogenous carcinogens or simply the process of aging. However, pediatric cancers do not arise as a result of aging or exogenous genotoxic agents. We are interested in the etiology of genome instability in pediatric cancers and the resulting genome-protective responses, also called DNA damage responses, that are activated. Our long-term goal is to identify factors within DNA damage response signaling pathways that represent therapeutic vulnerabilities in order to develop new treatment options for children with cancer.

Our current investigations are centered around two etiologies of endogenous DNA damage in leukemia: (1) APOBEC3 enzymes, DNA cytosine deaminases that are expressed at high levels in acute myeloid leukemia, and (2) MLL (KMT2A) gene rearrangements that drive infant acute lymphoblastic leukemia.

Projects:
1. Functional whole-genome knock out screen to evaluate synthetic lethality with APOBEC3A expression in leukemia.
2. Impact of APOBEC3A on DNA replication.
3. DNA damage responses elicited by MLL gene rearrangements in infant leukemia.
4. Impact of APOBEC3 enzymes on normal and malignant hematopoiesis.

Mentorship and Commitment to Diversity Statement:
The Green Lab strives to have an environment of scientific productivity, collegiality, congeniality, collaboration, and fun! We are proud to be home to a diverse group of trainees from varied scientific, geographic, ethnic, and social backgrounds. We believe that diversity improves us as a group and welcome others who feel the same. We aim to promote diversity and look forward to recruiting lab members from backgrounds that are underrepresented in science. In the Green Lab, we celebrate differences and learn from one another.

Selected Publications:

Green AM, DeWeerd RA, O’Leary DR, Hansen AR, Kulej K, Dineen AS, Szeto JH, Hayer KE, Garcia BA, Weitzman MD (2021). Interaction with the CCT chaperonin complex limits cytotoxicity from the APOBEC3A cytidine deaminase. EMBO Reports, 22(9):e52145.

Green AM, Weitzman MD. The spectrum of APOBEC3 activity: From anti-viral agents to anti-cancer opportunities. DNA Repair (Amst). 2019 Nov;83:102700. doi: 10.1016/j.dnarep.2019.102700. Epub 2019 Sep 13. Review.

Kitt E, Hayes M, Cárdenas AM, Green AM. Interpretation and management of positive anti-hepatitis B core antibody tests in immunocompromised pediatric patients. Transpl Infect Dis. 2019 Jun;21(3):e13074.

Chang YC, Graf E, Green AM. Invasive Curvularia Infection in Pediatric Patients With Hematologic Malignancy Identified by Fungal Sequencing. J Pediatric Infect Dis Soc. 2019 Mar 28;8(1):87-91.

Green AM, Budagyan K, Hayer KE, Reed MA, Savani MR, Wertheim GB, Weitzman MD. Cytosine Deaminase APOBEC3A Sensitizes Leukemia Cells to Inhibition of the DNA Replication Checkpoint. Cancer Res. 2017 Sep 1;77(17):4579-4588.

Green AM, Landry S, Budagyan K, Avgousti DC, Shalhout S, Bhagwat AS, Weitzman MD. APOBEC3A damages the cellular genome during DNA replication. Cell Cycle. 2016;15(7):998-1008.

Last Updated: 9/14/2021 12:56:47 PM

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