Amjad Horani, M.D.

Assistant Professor
Pediatrics
Allergy & Pulmonary
Cell Biology and Physiology

Molecular Cell Biology Program

  • 314-273-4874

  • horani_a@wustl.edu

  • horanilab.wustl.edu

  • cilia, PCD, airway, lung

  • Our lab studies airway and motile cilia biology and investigates the effects of mutations on cilia function

Research Abstract:

The airway is lined with microscopic, hair-like structures called cilia that sweep the airway in a rhythmic motion. Cilia are essential in defending the airway from infection and injury.

Cilia dysfunction causes significant disease as evidenced by the genetic syndrome, primary ciliary dyskinesia (PCD). PCD is the prototype of inherited motile ciliopathy, though secondary dysfunction of cilia can occur in common conditions such as asthma, COPD, and may contribute to idiopathic bronchiectasis.

Other types of cilia called sensory or primary cilia are important for other cellular functions, and are related to conditions such as kidney disease, hearing loss, and blindness.

The Horani lab research focuses on airway epithelial cell differentiation and regulation, with special interest in cilia assembly and how it relates to impaired mucociliary clearance and the biology of primary ciliary dyskinesia. The lab uses primary culture of human and mouse cells, and employs genetic manipulation methods to investigate the function of novel proteins involved in ciliogenesis.

Mentorship and Commitment to Diversity Statement:
I am committed to reducing inequities in the scientific field, as well as the medical field by promoting a diverse environment within the lab. I
believe that medical education, and the experience aspiring scientists receive in the lab, are essential in promoting diversity within the scientific community as well as the general community as a whole.

Selected Publications:

Puray-Chavez M, LaPak KM, Schrank TP, Elliott JL, Bhatt DP, Agajanian MJ, Jasuja R, Lawson DQ, Davis K, Rothlauf PW, Jo H, Lee N, Tenneti K, Eschbach JE, Mugisha CS, Vuong HR, Bailey AL, Hayes DN, Whelan SPJ, Horani A, Brody SL, Goldfarb D, Major MB, Kutluay SB. Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cell. bioRxiv. 2021.

Brennan SK, Molter D, Menezes M, Dunsky K, Leonard D, Lieu J, Hirose K, Hazan G, Horani A, Ferkol T, Brody SL. Implementation of a screening tool for primary ciliary dyskinesia (PCD) in a pediatric otolaryngology clinic. Int J Pediatr Otorhinolaryngol. 2020;142:110586.

Lee C, Cox RM, Papoulas O, Horani A, Drew K, Devitt CC, Brody SL, Marcotte EM, Wallingford JB. Functional partitioning of a liquid-like organelle during assembly of axonemal dyneins. Elife. 2020;9.

Horani A, Ferkol TW. Understanding Primary Ciliary Dyskinesia and Other Ciliopathies. J Pediatr. 2020.

Puray-Chavez M, Tenneti K, Vuong HR, Lee N, Liu Y, Horani A, Huang T, Case JB, Yang W, Diamond MS, Brody SL, Dougherty J, Kutluay SB. The translational landscape of SARS-CoV-2 and infected cells. bioRxiv. 2020.

Hawkins FJ, Suzuki S, Beermann ML, Barillà C, Wang R, Villacorta-Martin C, Berical A, Jean JC, Le Suer J, Matte T, Simone-Roach C, Tang Y, Schlaeger TM, Crane AM, Matthias N, Huang SXL, Randell SH, Wu J, Spence JR, Carraro G, Stripp BR, Rab A, Sorsher EJ, Horani A, Brody SL, Davis BR, Kotton DN. Derivation of Airway Basal Stem Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2020.

Gileles-Hillel A, Mor-Shaked H, Shoseyov D, Reiter J, Tsabari R, Hevroni A, Cohen-Cymberknoh M, Amirav I, Brammli-Greenberg S, Horani A, Kerem E, Breuer O. Whole-exome sequencing accuracy in the diagnosis of primary ciliary dyskinesia. ERJ Open Res. 2020;6(4).

Bustamante-Marin XM, Horani A, Stoyanova M, Charng WL, Bottier M, Sears PR, Yin WN, Daniels LA, Bowen H, Conrad DF, Knowles MR, Ostrowski LE, Zariwala MA, Dutcher SK. Mutation of CFAP57, a protein required for the asymmetric targeting of a subset of inner dynein arms in Chlamydomonas, causes primary ciliary dyskinesia. PLoS Genet. 2020;16(8):e1008691.

Last Updated: 5/11/2021 5:18:22 PM

Back To Top

Follow us: