Siling Du

Program: Immunology

Current advisor: Jonathan Kipnis, PhD

Undergraduate university: Nankai University, 2020

Enrollment year: 2020

Research summary
Dura mater immunity in neurodegeneration.

Graduate publications
Du S, Ou F, Drieu A, Xu EZ, Cheng Y, Storck SE, Mamuladze T, Cao J, Abduljawad N, Bhattarai B, Rustenhoven J, Mortimer N, Brioschi S, Nguyen K, Rodrigues PF, Smirnov I, Gibson D, Michael White J, Beatty W, DeNardo D, Li Q, Meers M, Han CZ, Sun N, Ginhoux F, Cella M, Colonna M, Kipnis J. 2026 Brain-engrafted monocyte-derived macrophages from blood and skull-bone marrow exhibit distinct properties. Neuron, 114(11):1986-2005.e9. PMCID: PMC13052357

Antonova AU, Loo HQ, Smirnov I, Gilfillan S, Halabi CM, Ponce J, Yan J, Chang HY, Randolph GJ, Vermi W, Cella M, Meers M, Ostuni R, Gate D, Nguyen AT, Cruchaga C, Hu S, Kipnis J, Colonna M. 2026 . Cell, ():Online ahead of print. PMCID:

Hu H, Lin PB, Zeng C, Li Y, Bosch ME, Emmerson JT, Sharma P, Ohara RA, Dong W, Wu T, Du S, Gao W, Jiang H, Yuan L, Bao X, Li S, Vomund AN, Erdmann-Gilmore P, Gu Y, Hu M, Nulman J, Miller TM, Yok. 2026 Priming of CD8+ T cells by peripheral dendritic cells exacerbates tau-mediated neurodegeneration. Nat Neurosci, ():Online ahead of print. PMCID:

Ou F, Liu TT, Du S, Chen J, Kraft M, Nam G, Bhattarai B, Shin H, Koch AR, Murphy TL, Murphy KM. 2026 ID2 secures cDC1 specification by antagonizing E proteins at a pleiotropic Zeb2 enhancer. Nat Immunol, 27(10):2043-57. PMCID: PMC13603453

Park JH, Abramishvili D, Davanzo GG, Silva R, Gu X, Du S, Lee DD, Zinselmeyer BH, Turner JS, Randolph GJ, Smirnov I, Kipnis J. 2026 Functional role of skull lymphoid structures in CNS immunosurveillance. Nature, 658(8134):196-204. PMCID: PMC13626955

Hamagami N, Kapadia D, Barclay KM, Huang Y, Abduljawad N, Cheng Z, McLaughlin L, Singhania D, Ding X, Yang J, Sun Z, Karra V, Du S, Bayguinov P, Yu G, Li YE, Gabel HW, Li Q. 2026 State-specific enhancer landscapes govern microglial plasticity. Immunity, 59(2):288-305.e11. PMCID:

Chen Y, Liu Y, Nguyen K, Wu J, Song S, Lin K, Rodrigues PF, Du S, Zhou C, Xiong K, Bosch M, Lin PB, Khantakova D, Wu S, Wu M, Yuede C, Holtzman DM, Colonna M. 2026 Targeting amyloid-β pathology by chimeric antigen receptor astrocyte (CAR-A) therapy. Science, 391(6789):eads3972. PMCID:

Brioschi S, Belk JA, Storck SE, Wu Y, Wang Z, Feng Z, van Olst L, Emery JL, Sudan R, Wu J, Du S, Genua M, La Terza F, Edwards AJ, Nguyen KM, Sanford J, Lee DD, Bugatti M, Federico AJ, Chen Y, Wang Z, Penati S, Rodrigues PF, Antonova AU, Loo HQ, Smirnov I, Gilfillan S, Halabi CM, Ponce J, Yan J, Chang HY, Randolph GJ, Vermi W, Cella M, Meers M, Ostuni R, Gate D, Nguyen AT, Cruchaga C, Hu S, Kipnis J, Colonna M. 2026 Brain perivascular macrophages regulate endothelial cell function via a cMAF-dependent transcriptional program in mouse and human. Cell, ():Online ahead of print. PMCID: PMC13546755

Lee DD, Telfer KA, Davis DL, Smyth LCD, Ravindran R, Czepielewski RS, Huckstep CG, Du S, Kurashima K, Jain AK, Kipnis J, Zinselmeyer BH, Randolph GJ. 2025 ADAPT-3D:accelerated deep adaptable processing of tissue for 3-dimensional fluorescence tissue imaging for research and clinical settings. Sci Rep, 15(1):31841. PMCID: PMC12397274

Mamuladze T, Zaninelli TH, Smyth LCD, Wu Y, Abramishvili D, Silva R, Imbiakha B, Verhaege D, Du S, Papadopoulos Z, Gu X, Lee D, Storck S, Perrin RJ, Smirnov I, Dong X, Hu S, Diamond MS, Pinho-Ribeiro FA, Kipnis J. 2025 Mast cells regulate the brain-dura interface and CSF dynamics. Cell, 188(20):5487-5498.e16. PMCID: PMC12380238

Chen J, Du S, Cheng W, Amrute JM, Kim MW, Ohara RA, Han J, Jo S, Kim L, Wang Z, Song H, Postoak JL, Kim S, Randolph GJ, Kipnis J, Murphy TL, Murphy KM. 2025 Transcription factor Maf promotes expression of repressor Zeb2 to drive microglia development in primitive hematopoiesis. Immunity, 59(1):48-59.e5. PMCID: PMC12854547

Kim K, Abramishvili D, Du S, Papadopoulos Z, Cao J, Herz J, Smirnov I, Thomas JL, Colonna M, Kipnis J. 2025 Meningeal lymphatics-microglia axis regulates synaptic physiology. Cell, 188(10):2705-2719.e23. PMCID: PMC12086007

Du S, Zhou Y, Li D, Lier J, Cella M, Tada M, Hamasaki H, Wu J, Cai Z, Orthmann-Murphy JL, Kakita A, Kipnis J, Bergner CG, Colonna M. 2025 Mutations in the human CSF1R gene impact microglia’s maintenance of brain white matter integrity. Nat Immunol, 26(7):1198–211. PMCID:

Du S, Kipnis J. 2025 Replacing microglia to treat a brain disease. Science, 389(6756):129-30

Smyth LCD, Xu D, Okar SV, Dykstra T, Rustenhoven J, Papadopoulos Z, Bhasiin K, Kim MW, Drieu A, Mamuladze T, Blackburn S, Gu X, Gaitán MI, Nair G, Storck SE, Du S, White MA, Bayguinov P, Smirnov I, Dikranian K, Reich DS, Kipnis J. 2024 Identification of direct connections between the dura and the brain. Nature, 627(8002):165-73. PMCID: PMC11254388

Gao W*, Kim MW*, Dykstra T, Du S, Boskovic P, Lichti CF, Ruiz-Cardozo MA, Gu X, Weizman Shapira T, Rustenhoven J, Molina C, Smirnov I, Merbl Y, Ray WZ, Kipnis J. 2024 Engineered T cell therapy for central nervous system injury. Nature, 634(8034):693-701. PMCID:

Ou F, Liu TT, Desai P, Ferris ST, Kim S, Shen H, Ohara RA, Jo S, Chen J, Postoak JL, Du S, Diamond MS, Murphy TL, Murphy KM. 2024 Optimization of the Irf8 +32-kb enhancer disrupts dendritic cell lineage segregation. Nat Immunol, 25(11):2043-56. PMCID: PMC12103213

Rustenhoven J, Pavlou G, Storck SE, Dykstra T, Du S, Wan Z, Quintero D, Scallan JP, Smirnov I, Kamm RD, Kipnis J. 2023 Age-related alterations in meningeal immunity drive impaired CNS lymphatic drainage. J Exp Med, 220(7):e20221929. PMCID: PMC10083715

Brioschi S, Belk JA, Peng V, Molgora M, Rodrigues PF, Nguyen KM, Wang S, Du S, Wang WL, Grajales-Reyes GE, Ponce JM, Yuede CM, Li Q, Baer JM, DeNardo DG, Gilfillan S, Cella M, Satpathy AT, Colonna M. 2023 A Cre-deleter specific for embryo-derived brain macrophages reveals distinct features of microglia and border macrophages. Immunity, 56(5):1027-1045.e8. PMCID: PMC10175109

Drieu A, Du S, Kipnis M, Bosch ME, Herz J, Lee C, Jiang H, Manis M, Ulrich JD, Kipnis J, Holtzman DM, Gratuze M. 2023 Parenchymal border macrophages regulate tau pathology and tau-mediated neurodegeneration. Life Sci Alliance, 6(11):e202302087. PMCID: PMC10415611

Drieu A, Du S, Storck SE, Rustenhoven J, Papadopoulos Z, Dykstra T, Zhong F, Kim K, Blackburn S, Mamuladze T, Harari O, Karch CM, Bateman RJ, Perrin R, Farlow M, Chhatwal J; Dominantly Inherited Alzheimer Network; Hu S, Randolph GJ, Smirnov I, Kipnis J. 2022 Parenchymal border macrophages regulate the flow dynamics of the cerebrospinal fluid. Nature, 611(7936):585-593. PMCID: PMC9899827

Wang S, Sudan R, Peng V, Zhou Y, Du S, Yuede CM, Lei T, Hou J, Cai Z, Cella M, Nguyen K, Poliani PL, Beatty WL, Chen Y, Cao S, Lin K, Rodrigues C, Ellebedy AH, Gilfillan S, Brown GD, Holtzman DM, Brioschi S, Colonna M. 2022 TREM2 drives microglia response to amyloid-β via SYK-dependent and -independent pathways. Cell, 185(22):4153-4169.e19. PMCID: PMC9625082

Mazzitelli JA, Smyth LCD, Cross KA, Dykstra T, Sun J, Du S, Mamuladze T, Smirnov I, Rustenhoven J, Kipnis J. 2022 Cerebrospinal fluid regulates skull bone marrow niches via direct access through dural channels. Nat Neurosci, 25(5):555-60. PMCID: PMC9081158

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