Kyunghee Choi, Ph.D.

Associate Professor
Pathology and Immunology

Developmental, Regenerative and Stem Cell Biology Program
Immunology Program
Molecular Cell Biology Program

  • 314-362-8716

  • 314-362-9045

  • 314-362-8888

  • 8118

  • BJCIH 8th floor, room 8302

  • kchoi@pathology.wustl.edu

  • Cell fate, transcription factors, hematopoiesis, vascular, development, regeneration, stem cells, angiogenesis, tumor

  • Hematopoietic, Vascular and Cardiac Development in Mammalian System

Research Abstract:

We study molecular mechanisms that regulate hematopoietic and vascular development and regeneration. We have expertise on hematopoietic, vascular and stem cell biology as well as stem cell applications and have made significant contributions to the understanding of how hematopoietic and vascular systems are established during embryogenesis. For developmental studies, we utilize an in vitro differentiation model of pluripotent stem cells, such as embryonic stem (ES) and induced pluripotent stem (iPS) cells, and early mouse embryos to address self-renewal, lineage commitment, and differentiation of the hematopoietic and vascular systems. So far studies have shown that hematopoietic cells, endothelial cells, smooth muscle cells and cardiomyocytes develop from a subset of mesoderm expressing FLK1, a receptor tyrosine kinase (FLK1 expressing mesoderm). We are currently focusing on several transcription factors including ETS transcription factor ETV2, SCL, and GATA2 that control commitment from mesoderm to FLK1 expressing mesoderm and from the FLK1 expressing mesoderm to hematopoietic and endothelial cells. For regeneration studies, we utilize bone marrow transplantation, 5-FU injury, hindlimb ischemia injury and tumor transplantation models to investigate mechanisms regulating hematopoietic and vascular regeneration. We also utilize a mouse model of rheumatoid arthritis to investigate hematopoietic stem cell traits in pathologic conditions. Our current projects include 1) transcriptional control of hematopoietic and vascular cell lineage development 2) directed differentiation and applications of hematopoietic and vascular progenitors from stem cells 3) hematopoietic regeneration mechanisms 4) vascular repair and regeneration mechanisms 5) cardiac injury and regeneration mechanisms 6) gene and cell therapy for vascular repair and regeneration 7) tumor angiogenesis and 8) myeloid lineage skewing and inflammation.

Selected Publications:

Zhao, H. and Choi, K. 2017. A CRISPR screen identifies genes controlling Etv2 threshold expression in murine hemangiogenic fate commitment. Nature Communications. 8(1):541. doi: 10.1038/s41467-017-00667-5.

Xu, C., Lee, T., Sakurai, N., Krchma, K., Liu, F., Li, D., Wang, T. and Choi, K. 2017. ETV2/ER71 regulates hematopoietic regeneration through hematopoietic stem cell proliferation. J. Exp. Med. 214(6):1643-1653.

Park, C., Lee, T., Bhang, S.H., Liu, F., Nakamura, R., Oladipupo, S.S., Pitha-Rowe, I., Capoccia, B., Choi, H.S., Kim, T.M., Urao, N., Ushio-Fukai, M., Lee, D.J., Miyoshi, H., Kim, B., Lim, D.S., Apte, R.S., Ornitz, D.M and Choi, K. 2016. Injury mediated vascular regeneration requires endothelial ER71/ETV2. ATVB. 36:86-96

Sumanas, S. and Choi, K. 2016. ETS Transcription Factor ETV2/ER71/Etsrp in Hematopoietic and Vascular Development. Current topics in Developmental Biology. 118, 77–112.

Liu, F., Li, D., Yu, L., Kang, I., Cha, M., Watson, D.K., Park, C., Wang, T. and Choi, K. 2015. Induction of hematopoietic and endothelial cell program orchestrated by ETS transcription factor ER71/ETV2. EMBO Reports 16(5):654-669.




Last Updated: 9/18/2017 9:56:24 PM

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