Daniel Kerschensteiner, M.D.
Ophthalmology and Visual Sciences
Developmental, Regenerative and Stem Cell Biology Program
Molecular Cell Biology Program
We would like to understand the principles that guide the assembly of neural circuits and to decipher the way they process information. Our lab focuses on the mammalian retina. We use transgenic and ballistic gene delivery to fluorescently label specific neurons and connections in this circuit, and follow their development using confocal and multi-photon imaging. In addition, we use light - the natural input to this circuit - to elicit signals and track the processing of visual information among successive neurons of the circuitry using patch-clamp and multi-electrode array recordings. By combining these approaches, we hope to identify features of the retinal circuit architecture that perform particular computations and characterize how they arise during development. We will then probe underlying mechanisms of circuit assembly and function through genetically targeted manipulations of specific cells in the retina.
Tien NW, Kim T and Kerschensteiner D. Target-specific glycinergic transmission from VGluT3-expressing amacrine cells shapes suppressive contrast responses in the retina. Cell Rep 2016 May 17;15(7):1369-75
Faits MC, Zhang C, Soto F and Kerschensteiner D. Dendritic mitochondria reach stable positions during circuit development. Elife 2016 Jan 7; 5:e11583
Tien NW, Pearson JT, Heller CR, Demas J and Kerschensteiner D. Genetically identified suppressed-by-contrast retinal ganglion cells reliably signal self-generated visual stimuli. J Neurosci 2015 Jul 29;35(30):10815-20.
Kim T, Soto F and Kerschensteiner D. An excitatory amacrine cell detects object motion and provides feature-selective excitatory input to ganglion cells in the mouse retina. Elife 2015 May 19; 4.
Pearson JT and Kerschensteiner D. Ambient illumination switches contrast preference of specific retinal processing streams. J Neurophysiol 2015 Jul;114(1):540-50.
Johnson RE and Kerschensteiner D. Retrograde plasticity and differential competition of bipolar cell dendrites and axons in the developing retina. Curr Biol 2014 24(19):2301-6.
Kerschensteiner D. Spontaneous network activity and synaptic development. Neuroscientist 2013 23(2):272-90.
Soto F, Watkins KL, Johnson RE, Schottler F, and Kerschensteiner D. NGL-2 regulates pathway-specific neurite growth and lamination, synapse formation and signal transmission in the retina. J Neurosci 2013 33(29):11949-59.
Akrouh A and Kerschensteiner D. Intersecting circuits generate precisely patterned retinal waves. Neuron 2013 79(2):322-34
Schwartz GW, Okawa H, Dunn FA, Morgan JL, Kerschensteiner D, Wong RO and Rieke F. The spatial structure of a nonlinear receptive field. Nat Neurosci 2012 15(11):1572-80.
Soto F, Ma X, Cecil JL, Vo B, Culican SM and Kerschensteiner D. Spontaneous activity promotes synapse formation in a cell-type-dependent manner in the developing retina. J Neurosci 2012 32(16):5426-39.
Morgan JL, Soto F, Wong RO, Kerschensteiner D. Development of cell type-specific connectivity patterns of converging excitatory axons in the retina. Neuron 2011 71(6): 1014-20.
Last Updated: 5/31/2016 11:27:57 AM
Paired Recordings and 2-Photon Imaging of Retinal Ganglion cells