Makedonka Mitreva, Ph.D.

Associate Professor
Internal Medicine
Infectious Diseases
Genetics

Computational and Systems Biology Program
Molecular Genetics and Genomics Program
Molecular Microbiology and Microbial Pathogenesis Program

  • 314-286-2005

  • 8501

  • 4444 Forest Park Boulevard, St. Louis Mo, 63105

  • mmitreva@wustl.edu

  • http://genome.wustl.edu/people/groups/detail/mitreva-lab/

  • Genomics of Infectious Diseases, Helminth Genomics, Human Metagenomics

  • Next-generation genomic and computational approaches to empower the study of infectious diseases and the human microbiome

Research Abstract:

There are two main threads to my current research.

1. We have a sustained commitment to study neglected tropical diseases (NTDs). There are 17 parasitic and bacterial infections cause blindness, anemia, malnutrition, growth and development stunting in children, and severe morbidity and mortality during pregnancy in women. These infections can also increase the risk of co-infection with HIV/Aids, tuberculosis and malaria. Their impact on health and socioeconomic status of the affected populations (mainly developing world) result in promoting and maintaining poverty, and as the United Nation secretary general recently stated “Poverty reduction and the elimination of NTDs go hand-in-hand”. Our work translates basic science advances in genomics into practical knowledge that contributes to control or elimination of helminth NTDs (including hookworm, ascaris and whipworm; and flukes, including major biological cancerogens). My lab takes advantage of next-generation genomic and computational approaches to study: i) taxonomically restricted and differentially represented pathways in parasites and their potential as targets for drug development, ii) helminth genetic variations in natural infections, iii) host response to parasite infections, iv) identification of candidate antigens for vaccine or serodiagnostis, v) molecular characterization of understudied parasite of global importance. Through comparative pan-omics systems biology analysis we have provided fundamental molecular information for these devastating parasites of major medical and global importance accelerating both basic and translational research.

2. The second area of my research is focused on the human microbiome. My current efforts are to use targeted and shotgun sequencing of metagenomic communities of microbes to understand their biology. To study metagenomic communities through shotgun sequencing, advances in computational approaches and tools are needed therefore I develop new and improve existing computational tools that will allow precise identification and comparison of taxonomic structure and functional capability of microbial communities in a robust fashion. We use those tools to identify genes, pathways, and organisms within communities and use this information to determine what is common and variant among healthy and disease states, leading to identification of members or functions that are associated with different physiologic disease states.

Selected Publications:

Choi YJ, Bisset SA, Doyle SR, Hallsworth-Pepin K, Martin J, Grant WN, Mitreva
M. Genomic introgression mapping of field-derived multiple-anthelmintic
resistance in Teladorsagia circumcincta. PLoS Genet. 2017 Jun 23;13(6):e1006857.
PMID: 28644839; PMCID: PMC5507320.

McNulty SN, Tort JF, Rinaldi G, Fischer K, Rosa BA, Smircich P, Fontenla S,
Choi YJ, Tyagi R, Hallsworth-Pepin K, Mann VH, Kammili L, Latham PS, Dell`Oca N,
Dominguez F, Carmona C, Fischer PU, Brindley PJ, Mitreva M. Genomes of Fasciola
hepatica from the Americas Reveal Colonization with Neorickettsia Endobacteria
Related to the Agents of Potomac Horse and Human Sennetsu Fevers. PLoS Genet.
2017 Jan 6;13(1):e1006537. PMID: 28060841; PubMed PMCID: PMC5257007.

Choi YJ, Tyagi R, McNulty SN, Rosa BA, Ozersky P, Martin J, Hallsworth-Pepin
K, Unnasch TR, Norice CT, Nutman TB, Weil GJ, Fischer PU, Mitreva M. Genomic
diversity in Onchocerca volvulus and its Wolbachia endosymbiont. Nat Microbiol.
2016 Nov 21;2:16207. PMID: 27869792; PMCID: PMC5512550.

Wang Q, Heizer E, Rosa BA, Wildman SA, Janetka JW, Mitreva M. Characterization of parasite-specific indels and their proposed relevance for selective anthelminthic drug targeting. Infect Genet Evol. 2016 Apr;39:201-11. PMID: 26829384; PMCID: PMC4789095.

Gao X, Tyagi R, Magrini V, Ly A, Jasmer DP, Mitreva M. Compartmentalization of functions and predicted miRNA regulation among contiguous regions of the Nematode Intestine. RNA Biol. 2016 Mar 22:0. PMID: 27002534.

McNulty SN, Strübe C, Rosa BA, Martin JC, Tyagi R, Choi YJ, Wang Q, Hallsworth Pepin K, Zhang X, Ozersky P, Wilson RK, Sternberg PW, Gasser RB, Mitreva M. Dictyocaulus viviparus genome, variome and transcriptome elucidate lungworm biology and support future intervention. Sci Rep. 2016 Feb 9;6:20316. PMID: 26856411; PMCID: PMC4746573.

McNulty SN, Rosa BA, Fischer PU, Rumsey JM, Erdmann-Gilmore P, Curtis KC, Specht S, Townsend RR, Weil GJ, Mitreva M. An Integrated Multiomics Approach to Identify Candidate Antigens for Serodiagnosis of Human Onchocerciasis. Mol Cell Proteomics. 2015 Dec;14(12):3224-33. PMID: 26472727; PMCID: PMC4762623.

Wang Q, Rosa BA, Jasmer DP, Mitreva M. Pan-Nematoda Transcriptomic Elucidation of Essential Intestinal Functions and Therapeutic Targets With Broad Potential. EBioMedicine. 2015 Jul 29;2(9):1079-89. PMID: 26501106; PMCID: PMC4587998.

Rosa BA, Townsend R, Jasmer DP, Mitreva M. Functional and phylogenetic characterization of proteins detected in various nematode intestinal compartments. Mol Cell Proteomics. 2015 Apr;14(4):812-27. PMID: 25609831; PMCID: PMC4390262.

Tang YT, Gao X, Rosa BA, Abubucker S, Hallsworth-Pepin K, Martin J, Tyagi R, Heizer E, Zhang X, Bhonagiri-Palsikar V, Minx P, Warren WC, Wang Q, Zhan B, Hotez PJ, Sternberg PW, Dougall A, Gaze ST, Mulvenna J, Sotillo J, Ranganathan S, Rabelo EM, Wilson RK, Felgner PL, Bethony J, Hawdon JM, Gasser RB, Loukas A, Mitreva M. Genome of the human hookworm Necator americanus. Nat Genet. 2014 Mar;46(3):261-9. PMID: 24441737; PMCID: PMC3978129.

Taylor CM, Wang Q, Rosa BA, Huang SC, Powell K, Schedl T, Pearce EJ, Abubucker S, Mitreva M. Discovery of anthelmintic drug targets and drugs using chokepoints in nematode metabolic pathways. PLoS Pathog. 2013;9(8):e1003505. PMID: 23935495; PMCID: PMC3731235.

For the full list of publications from Mitreva lab in PubMed see:
https://www.ncbi.nlm.nih.gov/pubmed/?term=mitreva+m+or+mitreva-dautova

Last Updated: 8/8/2017 11:38:28 AM

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