Christina L. Stallings, Ph.D.

Assistant Professor
Molecular Microbiology

Molecular Microbiology and Microbial Pathogenesis Program
Biochemistry, Biophysics, and Structural Biology Program
Immunology Program
Plant and Microbial Biosciences Program

  • 314-286-0276

  • 314-286-0277

  • 314-362-3203

  • 8230

  • MPRB 8210

  • stallings@WUSTL.EDU

  • http://stallingslab.wustl.edu/

  • bacteria, gene regulation, molecular genetics, microbiology, pathogenesis, transcription, infectious disease

  • Molecular pathogenesis of Mycobacterium tuberculosis

Research Abstract:

Research in the Stallings Laboratory seeks to better understand the incredible ability of Mycobacterium tuberculosis to withstand hostile conditions during infection and persist for the lifetime of the host.

M. tuberculosis infection begins when inhaled bacilli enter the airways and are immediately exposed to phagocytic cells of the innate immune system. Infection of naive alveolar macrophages and dendritic cells leads to a proinflammatory response and the recruitment of lymphocytes, monocytes, and fibroblasts to form a granuloma. Within the granuloma, the infected cells are activated to kill the intracellular bacteria by imposing an arsenal of stresses. Despite this onslaught of stresses, the bacteria are able to persist for the lifetime of the host, indicating that M. tuberculosis mounts a significant defense against the immune system.

We have identified a number of mycobacterial proteins that function as mediators of mycobacterial stress responses and are required for pathogenesis. Our lab continues to investigate the roles of these proteins during infection and how their activities can be inhibited in new chemotherapeutic strategies to treat mycobacterial infections. In addition, we are investigating the host responses necessary to control M. tuberculosis infection and prevent active tuberculosis disease.

Selected Publications:

Weiss, L.A. and Stallings, C.L.. 2013. Essential Roles for Mycobacterium tuberculosis RelMtb beyond the production of (p)ppGpp. Under revision for JBac.

Srivastava, D. B., Leon, K, Osmundson, J, Garner, A., Weiss, L. A., Westblade, L., Glickman, M. S., Landick, R., Darst, S. A., and *Stallings, C. L., and *Campbell, E.A.. 2013. Structure and function of CarD, an essential Mycobacterial transcription factor. PNAS. 110(31):12619-24. PMID: 23858468. (*Co-corresponding authors).

Roxane Tussiwand, Wan-Ling Lee, Theresa L. Murphy, Mona Mashayekhi, Jrn C. Albring, Ansuman T. Satpathy, Wumesh KC, Jeffrey A. Rotondo, Brian T. Edelson, Nicole M. Kretzer, Xiaodi Wu, Michael Behnke, Leslie A. Weiss, Samuel S.K. Lam, Cora T. Aurthur, Christina L. Stallings, L. David Sibley, Robert D. Schreiber, and Kenneth M. Murphy. 2012. BATF leucine zipper-dependent interactions with IRF factors mediate class switch recombination, TH17 development and a novel Batf3-independent pathway of CD8a+ cDC development. Nature. 490(7421):502-7. PMID: 22992524.

Weiss, L. A., Harrison, P. G., Nickels, B. E., Glickman, M. S., Campbell, E. A., Darst, S. A., and Stallings, C. L. 2012. The interaction of CarD with RNAP mediates Mycobacterium tuberculosis viability, rifampicin resistance, and persistence. JBac. 194(20):5621-31. PMID: 22904282.

Stallings CL, Chu L, Li, L X and Glickman MS. Catalytic and non-catalytic roles for the mono-ADP-ribosyltransferase Arr in the mycobacterial DNA damage response. PLoS One 2011 6(7):e21807. PMID: 21789183

Barkan D, Stallings CL and Glickman MS. An improved counterselectable marker system for mycobacterial recombination using galK and 2-Deoxy-Galactose. Gene 2011 470(1-2):31-6. PMID: 20851171

Stallings CL and Glickman MS. CarD: A new RNA polymerase modulator in mycobacteria. Transcription 2011 2(1):15-18. PMID: 21326904

Stallings CL and Glickman MS. Is Mycobacterium tuberculosis stressed out? A critical assessment of the genetic evidence. Microbes and Infection 2010 12(14-15):1091-101. PMID: 20691805

Stallings CL, Stephanou NC, Chu L, Hochschild A, Nickels BE and Glickman MS. CarD is an essential regulator of rRNA transcription required for Mycobacterium tuberculosis persistence. Cell 2009 138(1): 146-59. PMID: 19596241

Last Updated: 3/4/2015 9:20:01 AM

Back To Top

Follow us: