Sebla B. Kutluay, Ph.D.

Assistant Professor
Molecular Microbiology

Molecular Microbiology and Microbial Pathogenesis Program
Computational and Systems Biology Program
Immunology Program
Plant and Microbial Biosciences Program

Research Abstract:

Because human immunodeficiency virus 1 (HIV-1) has an RNA genome, it is no surprise that protein-RNA interactions play key roles both in the replication cycle of HIV-1 and in the host defense against HIV-1 infection. Research in our laboratory focuses on understanding the molecular details of how HIV-1 replication is regulated by these host and viral RNA-binding proteins, the identification of the RNA targets of these proteins, and the discovery of novel RNA-binding proteins that enhance or block HIV-1 replication. To this end, we employ tools of microbiology, molecular biology, biochemistry and next-generation sequencing-based approaches.

Selected Publications:

Bieniasz, P. D. and Kutluay, S. B. 2018. CLIP-related methodologies and their application to retrovirology. Retrovirology 15(1):35.

Williams, G. D., Townsend, D., Wylie, K. M., Kim, P. J., Amarasinghe, G. K., Kutluay, S. B., Boon, A. C. M. 2018. Nucleotide resolution mapping of influenza A virus nucleoprotein-RNA interactions reveals RNA features required for virus replication. Nat. Commun. 9(1): 465.

Madison, M. K., Lawson, D. Q., Elliott, J., Ozanturk, A. N., Koneru, P. C., Townsend, D., Errando, M., Kvaratskhelia, M., Kutluay, S.B. 2017. Allosteric integrase inhibitors lead to premature degradation of the viral RNA genome and integrase in target cells. Journal of Virology 91(17). pii: e00821-17.

Kessl, J. J.*, Kutluay, S. B.*, Townsend, D., Slaughter, A., Larue, R. C., Shkriabai, N., Bakouche, N., Fuchs, J. R., Bieniasz, P. D., Kvaratskhelia, M. 2016. HIV-1 integrase binds the viral RNA genome and is essential during virion morphogenesis. Cell (accepted, *equal contribution).

York, A.*, Kutluay, S.B.*, Errando, M., Bieniasz, P.D. 2016. The RNA binding specificity of human APOBEC3 proteins resembles that of HIV-1 nucleocapsid. PLoS Pathogens (accepted, *equal contribution).

Kutluay, S.B. and Bieniasz, P.D. 2016. Analysis of HIV-1 Gag-RNA interactions by in cells and virions by CLIP-seq. In V. R. Prasad & G. V. Kalpana (Eds.) Methods Mol Biol. 1354:119-31 (co-corresponding authors).

Kutluay, S.B., Zang, T., Blanco-Melo, D., Powell, C., Jannain, D., Errando, M., Bieniasz, P.D. 2014. Global changes in the RNA binding specificity of HIV-1 Gag regulate virion genesis. Cell 159(5): 1096-109.

Kane, M., Yadav, S., Bitzegeio, J., Kutluay, S.B., Zang, T., Wilson, S., Schoggins, J., Rice, C., Yamashita, M., Hatziioannou, T., Bieniasz, P.D. 2013. Mx2 is an interferon-induced inhibitor of HIV-1 infection. Nature 502(7472): 563-6.

Soll, S.J., Wilson, S.J., Kutluay, S.B., Hatziioannou, T., Bieniasz, P.D. 2013. Assisted evolution enables HIV-1 to overcome a high TRIM5α-imposed genetic barrier to rhesus macaque tropism. PLoS Pathogens 9(9):
e1003667.

Kutluay, S.B., Perez-Caballero, D., Bieniasz, P.D. 2013. Fates of retroviral core components during unrestricted and TRIM5-restricted Infection. PLoS Pathogens 9(3): e1003214.

Wilson, S.J., Schoggins, J.W., Zang, T., Kutluay, S.B., Jouvenet, N., Alim, M.A., Bitzegeio, J., Rice, C.M., Bieniasz, P.D. 2012. Inhibition of HIV-1 particle assembly by 2`,3`-cyclic-nucleotide 3`-phosphodiesterase. Cell Host Microbe 12(4): 585-97.

Last Updated: 9/27/2018 9:29:21 AM

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