Todd P. Margolis, M.D., Ph.D.

Alan A and Edith L Wolff Distinguished Professor and Chairman
Ophthalmology and Visual Sciences

Molecular Microbiology and Microbial Pathogenesis Program

  • 314 362-3724

  • 8096

  • margolist@vision.wustl.edu

  • The cellular and molecular mechanisms that regulate the establishment and maintenance of latent neuronal infection with herpes simplex virus (HSV).

Research Abstract:

The primary focus of the Margolis laboratory is research on the cellular and molecular mechanisms that regulate the establishment and maintenance of latent neuronal infection with herpes simplex virus (HSV). Ongoing research is aimed at documenting the role of both neuronal and viral gene expression in the establishment and maintenance of HSV latency. The ultimate goal of this work is to gain enough of an understanding about the regulation of HSV latent infection that therapeutic interventions can be devised to eliminate latent infection or prevent viral reactivation. A second line of investigation focuses on development and evaluation of molecular diagnostics for infectious eye disease. A third line of investigation focuses on the use of telemedicine to screen AIDS patients for CMV retinitis in developing countries.

Selected Publications:

Margolis TP, Imai, Yang L, Vallas V and Krause PR. HSV-2 Establishes Latent Infection In a Different Population of Ganglionic Neurons than HSV-1: Role of LAT. J Virol. 81:1872-8, 2007.

Margolis TP, Elfman F, Leib, D, Pakpour, N, Apakupakul, K,, Imai, Y, and Voytek C. Spontaneous Reactivation of HSV-1 in Latently Infected Murine Sensory Ganglia. J Virol. 81:11069-74, 2007.

Imai Y, Apakupakul K, Krause PR, Halford W, and Margolis TP. Investigation of the Mechanism by Which Herpes Simplex Virus Type 1 LAT Sequences Modulate Preferential Establishment of Latent Infection in Mouse Trigeminal Ganglia. J Virol. 83:7873-82, 2009

Bertke AS, Patel A, Imai Y, Apakupakul K, Margolis TP, Krause PR. Latency-associated transcript (LAT) exon 1 controls herpes simplex virus species-specific phenotypes: reactivation in the guinea pig genital model and neuron subtype-specific latent expression of LAT. J Virol. 83:10007-15, 2009.

Tang S, Bertke A, Patel A, Margolis TP and Krause PR. Herpes Simplex Virus-2 miR-H6 is a Novel LAT-associated MicroRNA, but Reduction of its Expression Does not Influence Viral Latency Establishment or Recurrence Phenotype. J Virol 85:4501-9, 2011.

Bertke AS, Swanson SM, Chen J, Imai Y, Kinchington PR, Margolis TP. A5-Positive Primary Sensory Neurons are Non-Permissive for Productive Infection with Herpes Simplex Virus 1 In Vitro. J Virol 85:6678-86, 2011.

Smits SL, Manandhar A, van Loenen FB, van Leeuwen M, Baarsma GS, Dorrestijn N, Osterhaus ADME. Margolis TP, Verjans GMGM. High Prevalence of Anelloviruses in Vitreous Fluid of Children with Seasonal Hyperacute Panuveitis. J Infect Dis. 205(12):1877-84. 2112.

Bertke AS, Apakupakul K, Ma A, Imai Y, Gussow A, Wang K, Cohen JI, Bloom DC and Margolis TP. LAT region factors mediating differential neuronal tropism of HSV-1 and HSV-2 do not act in trans. Plos One 7 (12):e53281, 2012.

Bertke A, Ma A, and Margolis TP. Different Mechanisms Regulate HSV-1 and HSV-2 Productive Infection in Adult Trigeminal Neurons. J. Virol. 87: 6512-16, 2013.

Tang S, Bosch-Marce M, Patel A, Margolis TP, and Krause PR, Characterization of HSV-2 primary miRNA transcript regulation J Virol. 89: 4837-48. 2015

Last Updated: 1/20/2016 12:15:41 PM

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