Benjamin D. Humphreys, M.D., Ph.D.

Associate Professor
Internal Medicine
Renal

Developmental, Regenerative and Stem Cell Biology Program

  • 314 362-8233

  • 314-362-4665

  • 314-362-8237

  • 8126

  • Wohl Clinic, 9th floor

  • humphreysbd@wustl.edu

  • http://humphreyslab.com/

  • https://twitter.com/HumphreysLab

  • Stem cells, regenerative medicine, injury, fibrosis, gene editing, iPS cells

  • Define cell hierarchies in kidney injury and repair and thereby identify the critical cell types in kidney repair and fibrosis

Research Abstract:

The mission of my laboratory is to identify novel therapeutic targets for patients suffering from chronic disease, particularly fibrosis. Our primary tools are mouse genetics, adult injury models, high-resolution imaging and iPS cell differentiation into kidney organoids. We apply CRISPR-Cas9 gene editing, RNA-sequencing including Drop-Seq

Over 100,000 people per year develop kidney failure in the US and most are treated with dialysis therapy. While life-saving, this procedure is costly, inconvenient and accelerates heart disease.

An average dialysis patient has a 1 in 5 chance of dying every year. Paying for dialysis costs 7 percent of the entire Medicare budget, even though these patients make up only 1 percent of the Medicare population.

In The Humphreys Lab we are developing new and innovative treatments to help patients with kidney disease. We are using human stem cells to generate kidney organoids in a dish, with a goal of one day transplanting them into patients with kidney failure. We also study the kidney’s ability to regenerate itself so that we can harness this ability for therapeutic uses.

Selected Publications:

Miyagi A, Lu, A and Humphreys BD. Brief Review: Gene Editing: Powerful New Techniques for Nephrology Research and Therapy. J Am Soc Nephrol. 2016.

O.H Maarouf, A. Aravamudhan, D. Rangarajan, T. Kusaba, V. Zhang, J. Welborn, D. Gauvin, X. Hou, R. Kramann and B.D. Humphreys. Paracrine Wnt1 drives interstitial fibrosis without inflammation by tubulo-interstitial crosstalk. J Am Soc Nephrol, 2016; 27(3):781-90.

R. Kramann, R.K. Schneider, D.P. DiRocco, F. Machado, S.V. Fleig, P.A. Bondzie, J.M. Henderson, B.L. Ebert and B.D. Humphreys. Perivascular Gli1+ progenitors are key contributors to injury-induced organ fibrosis. Cell Stem Cell, 16(1):51-66, 2015.

R. Kramann, S.V. Fleig, R.K. Schneider, S.L. Fabian, D.P. DiRocco, O.H. Maarouf, J. Wongboonsin, Y. Ikeda, D. Heckl, S.L. Chang, H.G. Rennke, S.S. Waikar and B.D. Humphreys. Gli2 regulates myofibroblast cell-cycle progression in kidney fibrosis and is a novel therapeutic target. J Clin Invest, 125(8):2935-51,2015.

R. Kramann, M. Tanaka and B.D. Humphreys. Fluorescence Microangiography for Quantitative Assessment of Peritubular Capillary Changes after Acute Kidney Injury in Mouse. J Am Soc Nephrol, 2014, 25(9):1924-31, 2014.

I. Grgic, A.M. Krautzberger, A. Hofmeister, M. Lalli, D.P. DiRocco, S.V. Fleig, J. Liu, J.S. Duffield, A.P. McMahon, B. Aronow and B.D. Humphreys. Translational profiles of medullary myofibroblasts during kidney fibrosis. J Am Soc Nephrol, 25(9):1979-90, 2014.

I. Grgic, A.F. Hofmeister, G. Genovese, A.J. Bernhardy, H. Sun, O.H. Maarouf, V. Bijol, M.R. Pollak and B.D. Humphreys. Discovery of new glomerular disease-relevant genes by translational profiling of podocytes in vivo. Kidney Int, 86(6);1116-29, 2014.

T. Kusaba, M. Lalli, R. Kramann, A. Kobayashi and B.D. Humphreys. Differentiated kidney epithelial cells repair injured proximal tubule. Proc Nat Acad Sci, 111(4):1527-32, 2014. PMCID: 3910580

D. DiRocco, J. Bisi, P. Roberts, J. Strum, K. Wong, N. Sharpless and B.D. Humphreys. CDK4/6 inhibition induces epithelial cell cycle arrest and ameliorates acute kidney injury. Am J Physiol: Renal, 306(4):F379-88, 2014. PMCID: 3920026

B.D. Humphreys, F. Xu, V. Sabbisetti, I. Grgic, S.M. Naini, N. Wang, G. Chen, S. Xiao, D. Patel, J.M. Henderson, T. Ichimura, S. Mou, S. Soeung, A.P. McMahon and J.V. Bonventre. Chronic epithelial kidney injury molecule-1 expression in mice causes inflammation, fibrosis and kidney failure. J Clin Invest, 123(9):4023-35, 2013.

Last Updated: 7/7/2016 9:29:46 AM

Humphreys Lab 2016
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